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Primer designing procedure/Help Report bugs/Request features Run this program locally Change log
Target selection
Select genome

Gene selected
bp upstream to 
bp downstream

Fragment selection parameters
and ? bp to bp
No | Yes
Annealing temperature parameters
Primer selection parameters
all combinations
specify primary and secondary enzymes
limit enzymes

Last updated Jan 25, 2018.

Initial primer designer code written by Alex Gileta under guidance of Scott Smemo and Noboru Sakabe. Definitive designer written by Noboru Sakabe. Web interface and service created, provided and maintained by Noboru Sakabe.
Nobrega lab - Department of Human Genetics - University of Chicago, Chicago, USA.

Accessibility tested with https://wave.webaim.org/ on Jan 28, 2021, no errors were found.